Mobilisation of local and subnational stakeholders is critical to reaching the highest ambition of the Post-2020 Global Biodiversity Framework. Research undertaken by the NATURVATION project reveals that many cities are already actively engaged in nature-based solutions. These subnational government actions resonate with the Post 2020 framework and should form the basis of any urban-focused commitment platform. City contributions to global biodiversity goals can be categorised as actions that Conserve nature, Restore nature, and mobilise people’s ability to Thrive with nature (or how nature contributes to society).